منابع مشابه
A Modified lentiviral vector construction system
Objective: To develop a lentiviral vector system, which can be used to co-express multiple genes of interest, such as siRNAs cassettes, reporter gene and resistant gene simultaneously and to facilitate to titrate lentiviral vector stock with TCID50 method. Method: Synthesis new multiple cloning sites(MCS)to replace the original MCS of pLKO-1-puro and produced an intermediate plasmid pLKO-1-puro...
متن کاملConstruction and verification of the targeted uPA-shRNA lentiviral vector and evaluation of the transfection and silencing rate
Urokinase-type plasminogen activator (uPA) receptors, which are released by the synovial tissue, are responsible for the activation of cartilage-breakdown proteases and play critical roles in cartilage degradation during the progression of osteoarthritis (OA). RNA interference (RNAi) technology has emerged as a potent tool to generate cellular knockdown phenotypes of a desired gene. The aims of...
متن کاملConstruction of a lentiviral vector containing shRNA targeting ADAM17 and its role in attenuating endotoxemia in mice
Systemic inflammatory response syndrome is a pathophysiological inflammatory response mediated largely by tumor necrosis factor‑α (TNF‑α), in response to infectious or non‑infectious stimuli. TNF‑α secretion in response to bacterial lipopolysaccharide (LPS) is regulated in part by disintegrin and metalloproteinase domain‑containing protein 17 (ADAM17). Therefore, the present study aimed to iden...
متن کاملconstruction of vector fields with positive lyapunov exponents
in this thesis our aim is to construct vector field in r3 for which the corresponding one-dimensional maps have certain discontinuities. two kinds of vector fields are considered, the first the lorenz vector field, and the second originally introced here. the latter have chaotic behavior and motivate a class of one-parameter families of maps which have positive lyapunov exponents for an open in...
15 صفحه اولLentiviral Vector
A retroviral vector system based on the human immunodeficiency virus (HIV) was developed that, in contrast to a murine leukemia virus-based counterpart, transduced heterologous sequences into HeLa cells and rat fibroblasts blocked in the cell cycle, as well as into human primary macrophages. Additionally, the HIV vector could mediate stable in vivo gene transfer into terminally differentiated n...
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ژورنال
عنوان ژورنال: North American Journal of Medical Sciences
سال: 2010
ISSN: 1947-2714
DOI: 10.4297/najms.2010.2598